Genetic Diversity and Differences among Three F1 Families and Two Wild Populations of Genus Scylla Using Microsatellite Markers

Genetic diversity is the determinant of allocation germplasm resources in genetic improvement aquaculture species. In this study, three F1 families, including a hybrid Scylla family (S. paramamosain ♂ × S. serrata ♀), paternal paramamosain, maternal serrata, and two wild populations, population were used to investigate difference. The results indicated that 98 alleles nine microsatellites loci observed five populations. highest average value Ho (observed heterozygosity), He (expected PIC (polymorphic information content) 0.790, 0.799, 0.771, respectively, suggesting has high diversity. comparative analysis PIC, Fst (fixation index), HWE (Hardy–Weinberg equilibrium) may be more suitable for artificial breeding than from perspective allele frequency. Analysis molecular variance (AMOVA) showed total variation mainly occurred within populations (73.28%), demonstrating induce differentiation groups Scylla. value, UPGMA (unweighted pair-group mean analysis) dendrogram, offspring had close distance identity with It potential affinities similar paramamosain. study will provide valuable evaluate difference structure between distinct parental